Such a large listing fee makes me think you added a Reserve to that listing?
These are the most expensive optional listing fee, costing 4% of your set reserve, and it's payable sale or no sale, and charged the second it goes live, even if you remove the item seconds / minutes later.
If you wish to contact eBay Customer Services to look into this for you, this is the easiest and quickest way to contact eBay Customer Services, for a Call Back option.
https://www.ebay.co.uk/help/eua?id=5275&mkevt=1&mkpid
Lines open between 8 a.m. to 10 p.m. on weekdays.
9 a.m. to 6 p.m. on weekends.
I recommend contacting CS very first thing in the morning, when the lines first open, as there's more chance of Dublin answering.
@stevewhite07