Reserve price??

I made a mistake by putting a Reserve price on an item I'm selling, after a while I removed this Reserve price, will I still be Charged £5.99 because it's still showing in my Bill. Thank you

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Yes. Reserves are payable even if you unlist the item, or if it doesnt sell. Reserves are expensive and non refundable, so it's far better to simply start your auction at a sensible, minimum price that you would be happy to accept.